Cryo-FIB Scientist
Job ID JR101198 Date posted 05/21/2024The National Cryo-Electron Microscopy (cryo-EM) Center, the Laboratory for BioMolecular Structure (LBMS) at Brookhaven National Laboratory, provides sample preparation, screening, and high-resolution data collection for both single-particle cryo-EM and cryo-electron tomography (cryo-ET) studies.
Position Description
To expand LBMS’s cryo-ET capabilities, we are establishing a cryo-focused ion beam (cryo-FIB) platform to prepare thin lamellae of cells/tissues. In combination with cryo-ET, biological interactions in cellular environment and the organization/morphology of living cells can be visualized.
The LBMS is seeking an experienced scientist to join our team and support our efforts in expanding cryo-ET capabilities. The successful candidate will have a strong background in biology, biochemistry, and experience with cryo-ET techniques.
Essential Duties and Responsibilities:
- Use cryo-focused ion beam (cryo-FIB) to prepare thin lamellae for cryo-ET.
- Use cryo-ultramicrotome to prepare thin sections of tissues for cryo-ET.
- Use high-pressure freezer to prepare thick samples.
- Collect cryo-ET data and perform data analysis.
- Support the operation of LBMS.
Required Knowledge, Skills, and Abilities:
- PhD in cell biology, plant biology, structure biology, immunology, biochemistry, biophysics, chemistry, cell biology, plant genetics, microbiology, virology, or a related field and at least 2 years postdoctoral experience.
- Proven abilities for dissemination of research through publication in peer-reviewed journals.
- Ability to communicate effectively and constructively.
- Ability to work with flexible scheduling.
- Self-motivated and able to work both independently and as part of a team.
Preferred Knowledge, Skills, and Experience in one or more of the following
- Experience in use of a cryo-focused ion beam (cryo-FIB) for milling biological samples.
- Experience in use of a ultramicrotomes to cut biological tissues.
- Experience in use of high-pressure freezer to freeze thick samples for cryo-FIB or ultramicrotome.
- Experience in use of SEM and fluorescence microscopy.
- Experience in cryo-ET technique including sample preparation, data collection and analysis.
Other Information:
- Review of applications begins immediately. Applications will be accepted until the position is filled.
- Candidates should submit a CV/resume (no more than 5 pages), a brief description of research interests in a cover letter and contact information for three references.
Brookhaven Laboratory is committed to providing fair, equitable and competitive compensation. This is a multi-level role and the full salary range for this position is $101200 - $184000 / year. You will be placed at the level and salary commensurate with your experience. Salary offers will be commensurate with the final candidate’s qualification, education and experience and considered with the internal peer group.
Brookhaven employees are subject to restrictions related to participation in Foreign Government Talent Recruitment Programs, as defined and detailed in United States Department of Energy Order 486.1A. You will be asked to disclose any such participation at the time of hire for review by Brookhaven. The full text of the Order may be found at: https://www.directives.doe.gov/directives-documents/400-series/0486.1-BOrder-a/@@images/file
Equal Opportunity/Affirmative Action Employer
Brookhaven Science Associates is an equal opportunity employer that values inclusion and diversity at our Lab. We are committed to ensuring that all qualified applicants receive consideration for employment and will not be discriminated against on the basis of race, color, religion, sex, sexual orientation, gender identity, national origin, age, status as a veteran, disability or any other federal, state or local protected class. BSA takes affirmative action in support of its policy and to advance in employment individuals who are minorities, women, protected veterans, and individuals with disabilities. We ensure that individuals with disabilities are provided reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment. Please contact us to request accommodation.
*VEVRAA Federal Contractor
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